الفهرس 
Title : Effect of some environmental toxicants on oxidative stress related genes in rats
AbstractOnly 14 pages are availabe for public view
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Abstract
Dietary components have recently received rapidly expanding
attention for their potential to halt or reverse the development of many oxidative
stress-mediated diseases after exposure to environmental toxicants. 7, 12
Dimethyl Benz(a)anthracene (DMBA) is one of the most common
environmental pollutants. The aim of the present study was to evaluate the
chemopreventive effects of Broccoli as nutritional components against DMBA
intoxication in rats. A daily dose of Aqueous and methanolic broccoli extracts
(1ml/Rat,150mg/kg) respectively were given to 50 days old female rats for 26
successive weeks after carcinogen intoxication with a single dose of (20mg/ml)
of DMBA. DMBA intoxication resulted in redox imbalance (decreased GSH
level and increased MDA level), and increased DNA damage fragmentation in
liver, kidney and brain and bcl2 gene in kidney and brain. Besides, it affected
the level of expression of GST gene in liver, kidney and brain tissue but not
affected cfos gene level of expression. The aqueous and methanolic broccoli
extract supplements ameliorated the adverse effects induced by DMBA
intoxication by increasing the level of GSH and decreasing the MDA level with
concomitant lowering in DNA fragmentation, decreased expression of GST and
bcl2 in liver and brain, respectively as well as up-regulated GST and bcl2
expression in kidney. Broccoli as dietary components had a strong
chemoprotective effect against oxidative stress, DNA damage and genotoxicity
induced by DMBA intoxication in rats.