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Abstract
Influenza virus is a globally important respiratory pathogen which causes a high degree of morbidity and mortality annually, Influenza virus co-infection with pneumococcus often leads to severe complications Viral bacterial co-infection accounts for substantial illness and death in seasonal and pandemic influenza. This study was undertaken to provide insights into influenza virus and bacterial effect confection by showing correlation with mortality. Two hundred and forty patients with ILI (influenza like illness) were collected from September 2011 to May 2014. Apanal of different influenza virus types and subtypes was screened by RT-PCR (real time polymerase chain reaction) according to CDC(centers for disease control and prevention) and pneumolysin gene was detected by convention PCR. Positive Influenza virus represented (20.83%) of all patients. Of the positive specimens, (12.92%) were A viruses, and (7.92%) were influenza B viruses. Among the influenza A viruses were subtyped, (51.6%) were seasonal influenza A (H3) viruses, and (48.4%) pandemic swine (H1N1) viruses. Co-infection with bacteria represented (28.39%) of all patients (p-value = 0.022). Mortality showed the highest prevalence in co-infected patient with bacteria (P-value <0.001) and specially in infants .In conclusion, Early and simultaneous identification of influenza viruses and other bacterial confection using real time PCR can be incorporated into the work flow of clinical laboratory and allows for the identification of disease in a timely and reliable manner, moreover to reduced rate of morbidity and mortality. Indeed, Influenza virus in Egyptian patients is still present and still threatened our life