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Abstract
G. intestinalis is one of the most common human enteric protozoan worldwide that affect both humans and animals. This cross-sectional study was aimed to characterize the Giardia genotypes isolated from the stool of symptomatic and asymptomatic Egyptians and compare them with demographic and clinical data. Single stool sample was collected from 389 individuals (245 complaining of gastrointestinal symptoms and 144 apparently healthy asymptomatic individuals). Microscopically 62 positive fecal Samples for G. intestinalis trophozoites and/or cysts from both symptomatic and asymptomatic cases. Positive samples were molecularly characterized by Copro-nPCR targeting beta-giardin gene, and nPCR products were analyzed by RFLP for assemblage identification. Giardia was a frequent pathogen (62/245{25.3%}) in both symptomatic (53/245{21.6%}) and asymptomatic (9/144{6.25%}). DNA of 52 from 62 (83.9%) microscopically positive samples was amplified by nPCR. There was assemblage B predominance in 43 cases (82.7%) in both study groups 36 (83.7%) of symptomatic and 7 of (16.3%) asymptomatic persons, while 9 (17.3%) samples had assemblage A. Among the studied variables, only flatulence was significantly associated with Giardia infection and assemblage B.