الفهرس يوجد فقط 14 صفحة متاحة للعرض العام
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المستخلص
The use of nitrofuran and nitroimidazole derivatives as veterinary drugs is banned according to european commission regulation 37 / 2010 and Egyptian standardization organization. LCMS / MS assay was developed and validated according to EU guidelines; CD 2002 / 657 / EC for determination of nitrofuran metabolites and nitroimidazole residues in honey. Crude samples were acid-treated in order to liberate matrix - bound residues. Modified QuEChERS protocol using acetonitrile extraction, without primary secondary amine followed by evaporation under vacuum was employed. Nitrofurantoin, furazolidone, furaltadone and nitrofurazone were determined via analysis of their metabolites AHD, AOZ, AMOZ and SEM, respectively while nitroimidazole residues; ronidazole (RNZ) and dimetridazole (DMZ) were determined directly. For all analytes, neat standard calibration curves, after correction for matrix effect were employed. Decision limit (CCÜ) and detection capability (CCÝ) were below the MRPL for nitrofurans (1.00 og Kg- 1) and the recommended concentration for nitroimidazole (3.00 og Kg- 1), respectively. The CCÜ, CCÝ, percentage recovery and CV% ranges were 0.12 - 0.74 og Kg⁻¹, 0.21-1.27 og Kg⁻¹, 90.96 - 104.80% and 2.65 - 12.58%, respectively. Assay performance was verified via analysis of the studied compounds in spiked, commercial honey samples previously tested PT sample (prawn sample) and a PT sample of honey. This work is part of the national initiative for establishing a monitoring program for drug residues in Egyptian honey