الفهرس Only 14 pages are availabe for public view
 |  | from | 114 |  |  |
| | | from | 114 | | |
Abstract
The current study was conducted to evaluate the effects of maturation time (30 or 40 hours) and effect of LH addition (10 æl/ml) to in vitro maturation medium on maturation rate (%) of dromedary camel oocytes. Also, to investigate the effect of using two sources of spermatozoa; epididymal spermatozoa (G1), frozen semen (G2). In addition, to study the effect of two insemination methods: conventional IVF and intracytoplasmic sperm injection (ICSI) on the cleavage rate and development competence of in vitro produced embryos as a trial to improve the reproductive efficiency of the dromedary camel. Cumulus oocytes complexes (COCs) were recovered from ovaries by slicing technique. Based on morphological characteristics (number of cumulus cells enclosed the oocytes and the homogeneity of the cytoplasm), only grade A and B oocytes were selected to be cultured in TCM- 199 medium for maturation at 38.5 {u1D52}C, 5% CO and 95% humidity in air for 30 or 2 40 hours according to maturation protocol. The mature oocytes were subjected to two sources of spermatozoa. For G1, a number of 205 oocytes were inseminated with fresh epididymal spermatozoa (1x106 spermatozoa/ml). In G2, a number of 290 oocytes were inseminated with frozen thawed semen (3x106 spermatozoa/ml). Whereas in ICSI group, 28 oocytes were denuded and inseminated (individually injected) by ICSI technique. The results showed a significantly (P{u2264}0.05) higher maturation rate (83%) in oocytes subjected to 30 h maturation compared to 40 h group (64%). On the other hand, the results showed no significant effect for LH addition between the two groups. There was no significant (P> 0.05) difference in cleavage and blastocyst rates between the two groups of sperm sources being (21%, 8.27% and 20.7%, 8.33%) for G1, G2 respectively. For fertilization techniques there was no significant (P> 0.05) difference in cleavage rate and blastocyst rate, between conventional IVF (20.9%, 8.3%) and ICSI (23%, 5.5%) respectively.