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Abstract
Three marketed products containing black cohosh, vitex extract and soy isoflavones for the treatment of menopausal symptoms were assessed biologically and analytically using different chromatographic techniques (UV, HPLC and HPTLC) and statistical analytical comparison between these methods were evaluated. A new suggested formula containing black cohosh extract in addition to other herbal extracts and fixed oils were evaluated biologically and the results revealed that this formula was more effective on inflammatory mediators and bone desorption markers than the marketed preparations. This formula was assessed analytically through fractionation were the lipophilic fraction containing fixed oils and vitamin E was analyzed by GLC and HPTLC, repectively, while the dichloromethane fraction containing royal jelly and valerian markers were assessed by HPLC and finally black cohosh and grape seed extracts were analyzed by HPTLC and UV analysis, respectively. On the other hand, four marketed fixed oil products for the treatment of premenstrual symptoms were qualitatively and quantitatively assessed through determination of their physical and chemical properties as well as assessment of their fatty acid profile by GLC. Two galactagogue herbal products containing oils of foenugreek, fennel and caraway as well as their powdered forms were also assessed biologically where the powder form was found to be more effective in increasing the lactogenic hormones. The analysis of these products involved the determination of pharmacopeial constants, microscopical examination, GLC analysis of fixed and volatile oils as well as determination of trigonelline in foenugreek powder by HPLC and HPTLC methods. Saponins in foenugreek were totally assessed by UV colorimetric reaction and identified individually by LC/MS. Diosgenin was also assessed by HPLC and HPTLC after hydrolysis of foenugreek sapononins. A new formula containing foenugreek, fennel, caraway, dill and cumin powders was biologically assessed and the results revealed its effectiveness rather than the marketed products.