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Abstract
The estimation of postmortem interval (PMI) is a critical issue in forensic investigations. There are multiple approaches commonly used to estimate the PMI including physical, biochemical, and entomological methods. These assessment methods are still unreliable and inaccurate. The decay of RNA after death seems to be rapid and time dependant that may be a useful tool for the PMI determination. This study aimed to evaluate the use of mRNA quantity as a possible indicator for PMI. Eighty - four adult female albino rats were divided into six groups. Rats were exposed after sacrifice to different environmental conditions. Liver, brain, skin and heart samples were extracted at 0, 24, 48 and 96 h postmortem. The mRNA of GAPDH gene of rats’ organs was quantitatively detected by real time RT - PCR There were significant negative correlations between GAPDH values and time intervals in the brain, heart and liver of dead rats left in ambient air at 30{u00BA} C and also rats buried in sand at ambient air 30{u00BA} C. There were significant negative correlations between GAPDH values and time intervals in the brain, heart and skin of dead rats submerged under water at ambient air 30{u00BA} C, and in the liver, heart and skin of dead rats left in air at 6{u00BA} C GAPDH mRNA could be a useful marker for the estimation of PMI