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Abstract
In the present study, fifty minced meat samples from butcher’s shops in three Egyptian Governorates were randomly collected in summer and winter seasons and analyzed for isolation of Staphylococcus aureus, Salmonella species, Escherichia coli and Listeria monocytogenes. The total bacterial counts were 9 x 10⁵ , 1 x 10⁶ CFU /g and the coliform counts were 7 x 10², 8 x 10² CFU /g, in winter and summer seasons respectively. S. aureus, E. coli, Salmonella spp., Listeria spp. and L. monocytogenes were isolated from 12(24%), 44 (88%), 3(6%), 9(18%) and0.0 (0%) samples. respectively. Further, 37(84.1%) of 44 E. coli isolates were belonged to the following serotypes (O1, O26, O125, O126, O146, O166, O18, O114, O151, O157, O158, O27, O78, O148, O159, O168 and O8), while 7 isolates (15.9%) were untypable with the available antisera. Detection of staphylococcal enterotoxins (A, B, C and D) in minced meat samples, the results showed that all S. aureus isolates gave negative for staphylococcal enterotoxins (A, B, C and D).The thirty seven E. coli isolates screened for the detection of verocytotoxin producing E .coli (VTEC). Results showed that all minced meat samples gave negative for VT (vt1, vt2 and eae) detection except one minced meat sample (E. coli O114 isolate) gave positive results for VT with percentage (2.7%)