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Abstract Little known is about the epidemiology of Cryptosporidium in fish. This study scopes out the prevalence and molecular detection of Cryptosporidium spp. in cat-fish (Clarias gariepinus). A total of 300 Carias gariepinus fish were collected from two fresh water sources; River Nile (180) and drainage canals (120). Stomach and intestine epithelium of each individual fish samples were screened with staining technique of modified Ziehl-Neelsen (mZN) for recognition of Cryptosporidium oocysts followed by Enzyme Linked Immunosorbent Assay (ELISA) for detection of Cryptosporidium antibodies and molecular characterization using complemented DNA polymerase chain reaction (cPCR). ELISA showed higher prevalence of 69.3% than that prevalence obtained by mZN, 64% for the total examined Clarias gariepinus fish. |