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العنوان
Biodiversity of Thermophilic Bacteria in Egyptian Hot
Springs /
المؤلف
Eman Khaled Ahmed
هيئة الاعداد
باحث / Eman Khaled Ahmed
مشرف / Mohamed Fayez Fouad Ibrahim
مشرف / Mona Hussein Sayed Badawi
مشرف / Hassan Moawad Abd Elaal
الموضوع
Agricultural Microbiology
تاريخ النشر
2022.
عدد الصفحات
98 p. :
اللغة
الإنجليزية
الدرجة
ماجستير
التخصص
الزراعية والعلوم البيولوجية (المتنوعة)
تاريخ الإجازة
15/5/2022
مكان الإجازة
جامعة القاهرة - كلية الهندسة - Agricultural Microbiology
الفهرس
Only 14 pages are availabe for public view

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from 97

Abstract

Geothermal water samples collected from Dakhla Oasis, Kharga Oasis, Pharaoh
Baths and Ras Sedr hot springs in Egypt were explored for the isolation of industrially
efficient thermostable amylase-, protease- and lipase- producers. Of 170 enzymeproducing isolates secured from colonies developed on agar media, 12 superior ones
were subjected to morphological characteristics and biochemical profiles. Cells appeared
cocci and spiral with the majority as bacilli. Adopting the Diagnostics GN/GP 24 (Ref.
1001, 1002), the tested candidates successfully utilized various substrates as carbon and
nitrogen sources besides their abilities to produce a number of exoenzymes. Six potent
amylase-, protease- and lipase-producing thermophiles, two for either, were further
identified by 16S rRNA gene sequencing. The amylase-producers were identified as
Aneurinibacillus thermoaerophilus and Bacillus licheniformis with respective similarity
percentages of 99.48 and 100.