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Abstract Enterococcus species represent part of the normal flora of the human gastrointestinal tract. However, recently they have gained greater interest due to their ability to emerge as one of the most important hospital acquired pathogens worldwide .They are implicated in urinary tract infections, endocarditis, wound infection, abdominal infections, pelvic infections, and bacteremia . Enterococci are intrinsically resistant to multiple antimicrobial agents; they show low-level resistance to β-lactams, resistance to cephalosporins, and low-level resistance to aminoglycoside. Therefore, rapid and accurate identification of enterococcal infections is crucial in the management and treatment for patients, also for prevention and control of VRE transmission. Our study focused on the conventional biochemical panel involved in identification of Enterococcus spp, the study included 308 samples sent for routine culture and sensitivity at microbiology lab form June 2020 to January 2021.Fifty isolates were selected randomly to perform automated identification through Vitek2 Compact system as well as conventional biochemical testing as PYR test and modified salt broth also antibiotic susceptibility were tested by disc diffusion method. Our study showed frequency of isolation of E.faecalis, E.faecium and E.gallinarum at 70%,26% and 4% respectively among the studied isolates. Pyrrolidonyl arylamidase (PYR)test was rapid and easy for interpretation. Modified salt broth together with PYR showed high accuracy. As regard antimicrobial susceptibility test, Penicillin showed the highest resistance among enterococcal species. |