الفهرس | Only 14 pages are availabe for public view |
Abstract Acinetobacter species resistance towards β-lactam antibiotics is mediated by different classes of β-lactamases production. In the present study, we attempted to detect class C (AmpC)-producing Acinetobacter isolates by two phenotypic methods (AmpC disk test with Tris- EDTA and DPT with APB test) and compare them with multiplex PCR as the gold standard method. The results of the study showed that the prevalence of AmpC-producing isolates was 68.8% by AmpC disk test, 72.9% by DPT with APB test, and 79.2% by PCR. The sensitivity of AmpC disk test and DPT with APB test were 86.8% and 92.1% respectively and the specificity was 100% for both tests. The most prevalent plasmid-mediated AmpC gene family was CIT. |