الفهرس | Only 14 pages are availabe for public view |
Abstract Thioacetamide (TAA) has been used extensively in the development of animal models of acute liver injury. Tinospora cordifolia belongs to the family Menispermaceae. The present study provides the evidence indicating the protective effect of the extract prepared from the dried leaves of Tinospora cordifolia on isolated rat hepatocytes and an animal model of hepatotoxicity. In the present study rat hepatocyte were isolated and treated with different doses of Tinospora cordifolia leaves extract. The multiple concentrations of ethanolic extract of tested plant was used and The maximum dose has No Observed Adverse Effect Level (NOAEL) equals (15.6 μg/g cell) were calculated from dose-response curve. The extract exhibited no significant cytotoxic activity against the isolated rat hepatocytes achieving a weak inhibitory activity under these experimental conditions with IC50 = > 500 μg/ml. Quantification of oxidative stress parameters in rat hepatocytes treated with Tinospora extract at dose (15.6 μg/g cell) . Untreated cells were used to quantify basal oxidation. The concentration of MDA was significantly lower (P<0.05) in TC-extract treated hepatocyte, as compared to control untreated. |