الفهرس 
The cell viability and functional activity of periodontal ligament fibroblasts isolated from periodontally affected human teeth as compared to healthy ones upon exposure to sesame oil : (In Vitro Study)
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Abstract
The aim of this study was to investigate the effect of sesame oil on PDLFs proliferation via MTT assay and level of bFGF expression using quantitative PCR analysis. Material and methods: PDLFs were isolated from the remaining periodontal tissues attached to the roots of donor human teeth after extraction, including periodontally normal teeth, free from inflammatory periodontal disease and indicated for extraction for orthodontic treatment in addition to hopeless teeth with chronic periodontitis indicated for extraction. A total of 16 human teeth were utilized in the experiment (n=16). A number of 8 human first premolars (n=8) with clinically healthy periodontium were collected from patients undergoing extraction for orthodontic treatment. PDLFs isolated from 8 teeth with health periodontium were randomly assigned to the following group: group 1 (Normal): Isolated periodontal fibroblast from four normal teeth (n=4). group 2 (Normal treated with oil): Isolated periodontal fibroblast from four normal teeth treated with 2.5 μl of oil/ml of media (n=4). A number of 8 teeth (n=8) diagnosed with chronic periodontitis considered hopeless and indicated for extraction was collected and PDLFs were isolated and randomly assigned into the following groups: group 3 (Periodontitis): Isolated periodontal fibroblast from four teeth with periodontitis (n=4). group 4 (Periodontitis treated with oil): Isolated periodontal fibroblast from four teeth with periodontitis treated with 2.5μl of oil/ml of media (n=4)