الفهرس 
INTRODUCTIONOnly 14 pages are availabe for public view
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Abstract
In this study the aim was to detect RVFVand MERS-CoVfrom imported camels from Sudan by using probe based quantitative PCR protocol to evaluate the current situation of these diseases on our borders as these diseases may be considered as a threat to our country for both animals and human health.
A total of 1103 samples were collected from imported camels at Al-Shalateen Quarantine from 21 herds as a representative sample from 11014 camels(10%) introduced to the quarantine for about one year. Blood samples and nasal swabs were pooled (10 sample / pool) and examined for the presence of RVFV and MERS-CoVrespectively by using quantitative real time PCR. All samples taken were negative for both viruses which indicate that there was no shedding of the virus at the period of investigation. Moreover the negative results may be due to the long distance between the two quarantines and continues vaccination of alternative speciesliving with camels with RVF vaccinewhich may reduce the chance for infection.
In this study, 921 camels were tested for brucellosis. The obtained results revealed that the prevalence of brucellosis among examined 921 camels was 84(9.1%) for BAPAT, 73(7.9%) for RBPT, 78 (8.5%) for i-ELISA. In addition, CFT confirmed 48 (57.1%) samples.
In addition, confirmed positive samples showed that percent of reactors was higher on mature camels than immature camels. Also, it was higher on females than males; these results indicated that brucellosis is a disease of adult mature animals mainly females.
In this study, trials were done to isolate brucella species from lymph nodes (from serologically positive she camels slaughtered at Al-Shalateen quarantine), lung, liver, spleen and stomach content of three aborted camel foeti. Brucella melitensis biovar 3 were isolated from stomach content culture of the three aborted foeti while our trails to isolate brucella species from lymph nodes, lung, liver, spleen were failed.
Brucella melitensis affects mainly sheep and goat. Such inter-species transmission situation may be the outcome of close contact between (sheep and goats) and camels.