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Abstract The thesis deals with the problem of the spread of microbes and antibiotic resistant genes through wastewater and sewage plants. Traditional methods have limited ability to assess the proportions of microbiome as well as antibiotic resistance genes. The application of modern research methods such as metagenome was tested to track those microbes and genes in the wastewater treatment plant and their extent in the treated water receiving environment. The results of this thesis showed the importance of next-generation sequencing analysis for environmental monitoring of wastewater treatment systems using metagenomics. These methods predict the efficiency of wastewater treatment process and track the spread of microbes and antibiotic resistance genes through the system. |