الفهرس 
Role of serum tryptase level as a mast cell biomarker in evaluating patients with chronic kidney disease
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Abstract
Background: Tryptase, is Mast cell (MC)-specific protease. Detection of tryptase above healthy levels in the blood or urine of a chronic kidney disease (CKD) patient would be a non-invasive biomarker of MC activity
Objective: To determine the role of Mast cells in facilitation of inflammation and fibrosis in chronic kidney disease (CKD) patients by serum tryptase level.
Patients and methods: 90 patients with chronic kidney disease from El kasr-El-aini hospital were included. Serum tryptase was measured at recruitment by sandwich ELISA immunoassay.
Results: The mean age of patients was 50.86 ±12.07 years ranging between 20 and 66 years. Male percent was 51.1%. Three (3.3%) patients had stage I CKD , 6 (6.7%) patients was stage II CKD , 1 (1.1%) patient had stage IIIa CKD , 9 (10.0%) patients had stage IIIb CKD, 11 (12.2%) patients had stage IV CKD, 26 (28.9%) patients had stage V CKD , and 34 (37.8%) patients were on regular dialysis. . Mean serum level of creatinine was 5.88±3.46 mg/dL ranging between 0.50 and 13.60 mg/dL with a median of 5.90 mg/dL. Mean serum level of urea was 132.69±58.51mg/dL ranging between 18 and 269 mg/dL with a median of 124 mg/dL. Mean albumin-to-creatinine ratio was 2787.84±4864.26 mg/mmol ranging between 30 and 30514 mg/mmol with a median of 996.5 mg/mmol. Mean glomerular filtration rate estimated by MDRD equation was 20.49±26.50 ml/min/1.73m2 ranging between 2.9 and 136.40 ml/min/1.73m2 with a median of 9.35 ml/min/1.73m2. While mean Serum tryptase serum level was 4.55±6.55 μg/L ranging between 0.05 and 20 μg/L with a median of 1.51 μg/L. We found that serum tryptase levels were higher among patients with CKD stage V when compared with other groups. Yet, the difference was not significant. Serum tryptase level was inversely correlated with age, albumin-to-creatinine ratio and glomerular filtration rate. While it was positively correlated with serum creatinine level and serum urea level. Yet, no relation was significant. Serum tryptase level was significantly lower among diabetic patients when compared with non-diabetic patients (2.82±5.53 vs5.87±7.00; p=0.002*).
Conclusion: elevated serum tryptase concentration is associated with stage III . IV CKD patients on regular dialysis more than those in stage I ,II CKD , yet ,no relation was significant