الفهرس 
Heavy metals
Ethylene diamine tetra acetic acid (EDTA)Only 14 pages are availabe for public view
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Abstract
Heavy metals are common contaminants that have negative impacts on the body’s organs and systems. Date (Phoenix dactylifera) has great interest in biomedical applications and traditional medicine for management of several diseases. This study evaluated the impact of Phoenix dactylifera seeds extract (PDSE) on cadmium (Cd)-induced hepatotoxicity in mice. Sixty mice were equally divided into six groups, G1 was negative control, G2 was injected i.p. with PDSE (300 mg/kg b.wt) daily, G3 was injected i.p. with Cd (6.5 mg/kg b.wt) daily, G4 was injected with Cd and PDSE as in G2 and 3, respectively. G5 was injected i.p. with EDTA (25 mg/kg b.wt). G6 was injected with Cd as in G3 and then with EDTA (25 mg/kg b.wt). On day 15, sera samples were collected for biochemical parameters assessment. Liver tissues were collected for determination of oxidants/antioxidants biomarkers, gene expression analysis, and for histopathological investigations. The results showed that treatment with PDSE significantly ameliorated the hepatic dysfunctions in the Cd-intoxicated mice that evidenced by significant improvement in the ALT, AST, ALP, and total protein levels as well as in the total bilirubin and GGT levels. Furthermore, there was a significant increase in the SOD and CAT activities with significant decrease in MDA levels after treatment of Cd-injected mice with PDSE. Treatment with PDSE significantly restored the alterations in the gene expression of the proinflammatory biomarkers and in the hepatic architectures that were induced by Cd injection. PDSE showed promising metal chelating activities in vitro and in Cd-intoxicated mice by ameliorating biochemical and histopathological alterations in the liver tissues that induced by Cd in male mice.