الفهرس 
Antimicrobial efficacy of a high-power diode laser and photo dynamic therapy using chitosan photosensitizer against enterococcus faecalis : An in-vitro study
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Abstract
Introduction: Complete eradication of microorganisms from the space of root
canal is the main goal for the successful root canal treatment and the health of
periradicular tissue .
Objective: To assess the antibacterial efficacy of sodium hypochlorite
(NaOCl) and 980 nm diode laser and PDT with chitosan on infected root
canals with Enterococcus ”E” Faecalis biofilms.
Material and Methods:
One hundred and five upper anterior incisors human teeth with single root and
root canal were instrumented by protaper system up to F3. Biofilms of E.
faecalis were generated based on a previously established protocol. Five teeth
were used to check the biofilm formation, then the remaining teeth were
randomly divided into two lines according to the disinfection methods; line A
:high power diode laser ,line B: photodynamic therapy. The antibacterial
efficacy was evaluated by colony forming units, and relative intensity of
live/dead bacteria, using Confocal Laser Scanning Microscopy . Data were
analyzed by proper statistical analysis with P= 0.05.
Results: Regarding the disinfection method, all groups in two lines had a
significantly high percentage of bacteria reduction (P<0.05). However, the
highest bacterial reduction was achieved with the combination of 2.5% NaOCl
and diode Laser followed by the combination of 2.5% NaOCL and PDT
(P<=0.001).
Conclusion: Under the conditions of the present study, results reinforced
that 980 nm diode laser irradiation is a useful adjunct in disinfection of
infected root canals with E. Faecalis biofilms.