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Abstract Background: Hydroxamate siderophores are low molecular weight, high affinity ferric iron- chelating compounds which are excreted by Aspergillus fumigatus for iron transport extracellularly or iron storage intracellularly. Methods: Aspergillus fumigatus was isolated from different samples , then siderophores were extracted from A. fumigatus by phenol, diethyl ether and purified by amberlite XAD-16 resin. Chemical structure of siderophores was described by H¹NMR , ESI-MS/MS and LC-MS/MS analysis. Also, the antimicrobial activity was observed. Results: from the elution profile of siderophores, the major peak was at fraction 28 (absorbance 0.98). As a result of H¹NMR analysis, the singlets which assigned to the -C=O-CH=C(CH3)C-substructure were detected .The siderophore TafC had antibacterial activity against both G +ve and G -ve bacteria. Conclusion: By using different concentrations of iron, it can be deduce that mycelial fresh weight positively affected by the concentration 0.03 mmol of iron at 20 h incubation period ,subsequently consequently production of siderophores increased. from chemical analysis, it was found that the chemical formula C39H58FeN6O15 was identical to the siderophore N,N’,N”- triacetylfusarinine C (TafC) which is known. |