الفهرس 
Effect of glycolic acid as single irrigant versus sodium hypochlorite and edta on enterococcus faecalis count and smear layer removal in single rooted teeth An In vitro study
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Abstract
Aim: The study aimed to compare 10% Glycolic acid as a single irrigant versus 2.5% sodium
hypochlorite followed by 17% EDTA in terms of E. faecalis count reduction and smear layer removal
in single-rooted teeth.
Methodology: Thirty human extracted mandibular premolars were prepared and inoculated
with E. faecalis for one week to evaluate E. faecalis count reduction. Teeth were randomly assigned
to two groups (n = 15) according to the irrigation protocol used. group I: intervention (10% Glycolic
acid) and group II: Control group (Irrigation using 2.5 % NaOCl followed by 17% EDTA). After 24
hours of incubation, bacterial count reduction using CFUs/ml was determined. For evaluating the
ability of irrigants to remove the smear layer, another twenty-two mandibular premolars were chosen
and randomized into two groups (n = 11). During chemo-mechanical preparations, the root canals
were irrigated with different irrigation protocols according to each group. Then, the roots were split
longitudinally in the bucco-lingual direction into two halves. ESMS at 1000x magnification was used
to evaluate the presence of a smear layer at the coronal, middle, and apical thirds of the root canal.Results: Regarding E. faecalis count reduction, there was no significant difference between
the two groups (p = 0.14). Regarding the ability to remove the smear layer, there was no significant
difference between the two groups at coronal, middle and apical. While comparing smear layer scores
within the same group, the apical third showed a significantly higher smear layer score in the two
groups.
Conclusion: Glycolic acid can be used as a single irrigant owing to its antibacterial efficacy
and the ability to remove the smear layer.