الفهرس 
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Abstract
The native breed of chicken is one of the most income-producing
species in the poultry sector in the Egyptian governorate of Ismailia.
Thus, the objective of the current study was to identify the collected
parasites using a light microscope and estimate the prevalence and
seasonal dynamics of the collected helminths using the newly introduced
molecular biology methods. 120 chickens out of 170 recorded (70.59%)
prevalence of helminth infestation during the period from September
2021 until the end of September 2022. four nematodes with a prevalence
(of 44.12 %) which were Ascarida galli, Heterakis gallinarum, Subulura
brumpti, Trichostrongylus tenuis, and four cestodes with (26.47 %)
which were Raillietina tetragona, R. echinobothrida, Hymenolepis
carioca, and Choanotaenia infundibulum. Eimeria spp. infestation
(11.18%), which were E. tenella, E. maxima, E. mitis, and E. burnette.
Ectoparasites (15.88%) were Echidnophaga gallinacean, Lipeurus
caponis, Menapon gallinae, Colombicola columbae, and Dermanyssus
gallinae. The identities of the certainly recovered nematode and cestode
species were confirmed by the blast test using DNA sequence data. Thus,
it is advised to use the molecular approach as the primary methodology
for the accurate identification of helminths, particularly in closely related species.