الفهرس 
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Abstract
Labile iron contributes significantly to oxidative stress. It reacts with hydrogen peroxide in Fenton reaction leading to ROS production. Oxidative stress is one of the factors taking part in the pathophysiology of atopic dermatitis. It upregulates the pro-inflammatory cytokines and cause oxidative damage of the protein in stratum corneum.
Atopic dermatitis is a chronic pruritic skin disease with an infancy or an early childhood onset. It is characterized by dry scaly skin with intense itching. Skin barrier dysfunction which includes disruption of fillagrin protein has been thought to represent the initial stage of the onset of atopic dermatitis. Genetic predisposition and immune dysregulation also play an important role. T- helper 2 cells, mast cells and basophils are the main cells incorporated in this disease. Th2 cells secrete cytokines of which IL4 and IL13 are the most important. This leads to activation of B lymphocytes with secretion of IgE which binds to its receptors on mast cells resulting in its degranulation with the release of inflammatory mediators like histamine and leukotrienes that mediate the allergic reaction.
Many mouse models have been used for studying atopic dermatitis. One of which is oxazolone induced atopic dermatitis model. Oxazolone is a hapten that can penetrate the skin, combine with protein and form a complex leading to activation of antigen presenting cells and Th2 immune response, a picture resembling human atopic dermatitis.
Iron chelators are compounds used as a treatment of transfusional iron overload. However, the new era is the use of iron chelating agents in the treatment of oxidative stress-related diseases. Desferrioxamine is the most popular iron chelator. It forms stable complex with iron and prevents it from reacting with peroxides in the environment. This complex is then eliminated in bile and urine.
The present study is aimed to explore the effect of iron chelation on the immunological parameters: skin interleukin-4 and serum immunoglobulin E to evaluate the role of iron in atopic dermatitis.
The present study was carried on 36 female CD1 mice aged 3-4 weeks, with body weights of approximately 20-30 grams. The period of the experiment was 4 weeks.
Mice were divided into 3 equal groups (12 mice each):
• Mice in control group received 20 µL of acetone (vehicle) with a pipette on both sides of the right ear.
• AD and DFO groups received 20 µL of 0.8% oxazolone dissolved in acetone with a pipette on both sides of the right ear.
• After one week, mice in