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Abstract This study was conducted to evaluate the appropriate methods and techniques for retrieval of two different lengths of a separated reused ProTaper Rotary Nickel titanium F2 files from root canal of mandibular premolars. This was followed by evaluation for success of retrieval and number of fragments retrieved within each method, the amount of removed dentin linearly and volumetrically after successful retrieval and the operating time. Eighty mandibular premolars were selected and classified in to two groups, each group comprised 40 samples according to the length of the separated fragments, group A (long fragments) and group B (short fragments). Each group was again classified in to two subgroups, each comprised 20 samples according to the method of retrieval, subgroup I utilized ultrasonic technique and needle-and-wire method whereas, subgroup II utilized ultrasonic technique and chloroform- dipped gutta-percha. The retrieval process was carried out by creating a circumferential staging platform, on the 40 short fragments, using modified Gates Glidden Drills |