الفهرس 
The evolution of shiga toxins of enterohemorrhagic escherichia coli (EHEC) and possible application in reverse vaccinology
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Abstract
This study was carried out to understand the possible role of phages in horizontal gene transfer in E. coli O157 and other enteric pathogens through in slico investigation of role of HGT in evolution of Shiga toxin and its expression among Enteric pathogens. The result of search of E. coli O157:H7 Shiga toxin gene sequences of clinical isolate that were retrieved by using NCBI BLAST alignment on database of NCBI using built in Mega5 software, showed 98-100% homologous sequences to the Shiga toxins to the retrieved genome sequences of Acintobacter hemolyticus, Citrobacter freundi, E.cloacae, Shegilla dysenteria, enteropathogenic E. coli and enteroaggregative haemorrhagic E .coli in addition to different types enterohaemorrhagic E .coli. This homologous percent result was confirmed by using the bioinformatics programs software for alignment and comparative gene sequence analysis; Mauve (Multiple genomes alignment analysis), MUMmer v3.20 (Multiple sequence alignment analysis) and CoCoNUT (Comparative sequence analysis)