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العنوان
Evaluation of quantitative immunomagnetic bead ELISA technique using super-paramagnetic nanoparticles in diagnosis of schistosomiasis haematobium /
الناشر
Kamal Eldin Mohammed Mokbel Kamal Eldin ,
المؤلف
Kamal Eldin Mohammed Mokbel Kamal Eldin
هيئة الاعداد
باحث / Kamal El-Din Mohammed Mokbel Kamal El-Din
مشرف / Abd El-Hamid Abd El-Tawab Sabry
مشرف / Mona Mahmoud Aly
مشرف / Ibrahim Rabia Baiuomy
تاريخ النشر
2014
عدد الصفحات
202 P. :
اللغة
الإنجليزية
الدرجة
ماجستير
التخصص
الطب (متفرقات)
تاريخ الإجازة
1/1/2014
مكان الإجازة
جامعة القاهرة - كلية الطب - Parasitology
الفهرس
Only 14 pages are availabe for public view

from 221

from 221

Abstract

Schistosomiasis haematobium is a serious public health problem in Egypt. Detection of S. haematobium antigens is a better immunodiagnostic tool than determination of the antibody level. We developed a novel immunomagnetic bead ELISA based on IgG for detection of CL antigen in sera and urine of patients infected with S. haematobium. Detection of CL Ag in serum using IMB-ELISA gave a sensitivity of 95%, a specificity of 93.3% compared to other parasitic infections group and 100% compared to healthy control group. While, detecting the same Ag in serum using sandwich ELISA gave a sensitivity of 85%, a specificity of 88.3% compared to other parasitic infections group and 100% compared to healthy control group. On the other hand, Detection of CL Ag in urine using IMB-ELISA gave a sensitivity of 91.6%, a specificity of 93.4 % compared to other parasitic infections group and 100% compared to healthy control group. While, detecting the same Ag in urine using sandwich ELISA gave a sensitivity of 83.3 %, a specificity of 81.8 % compared to other parasitic infections group and 100% compared to healthy control group