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Abstract
A total of 120 samples from luncheon , beef burger , kofta and minced meat were collected from different supermarkets in EL-Gharbia governorate for microbiological examination .The results showed that incidence of staphylococci was 49.6% , out of 120 samples 59 samples were positive with an incidence of 53.33% in luncheon , 33.33% in beef burger , 50% in kofta and 60% in minced meat .the incidence of staphylococcus aureus in luncheon , beef burger , kofta and minced meat were 10%,6.67%, 6.67% and 10% respectively .By using Polymerase Chain Reaction technique (PCR) For detection of ( sea, seb,sec, sed and see genes )The obtained results showed that 7 of 10 Staphylococcus aureus isolates were entertoxigenic , 5 of 7 obtained strain were positive for sea gene, and only 2 were positive for seb gene