الفهرس 
Genetic transformation in strawberry to produce virus resistant plantsOnly 14 pages are availabe for public view
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Abstract
Strawberry (Fragaria ananassa) is an economically important soft fruit crop with polyploidy genome, which makes the breeding of new cultivars difficult. Simple and efficient method for transformation and regeneration is required for cultivar improvement in strawberry. In the present study, adventitious shoot regeneration has been investigated in three cultivated strawberry varieties, i.e., festival, sweet charlie and florida via direct organogenesis protocol using the in vitro juvenile leaves as explants. To select the suitable organogenesis Protocol, the explants of the three cultivars were cultured on MS medium supplemented with different concentrations of TDZ (1, 2 and 3 mg / l), and incubated at a temperature of 22{u00B0}C ± 2. Medium containing 2 mg / l TDZ revealed the best regeneration efficiency with the three cultivars (73.3% for cultivar sweet charlie, and 72.6% for both cultivars festival and florida). After 4 weeks, the produced shoots were cultured on MS medium with different concentrations of BA and Kin to enhance shoots elongation. Results showed that the medium SE6 containing 1.5 mg / l BA and 0.5 mg / l Kin was the best for cultivars festival and sweet charlie, while, medium SE5 containing 1.5 mg / l BA and 0.1 mg /l Kin was the best for the cultivar Florida. Elongated shoots were successfully rooted by incubating the shoots on MS medium containing 1.5 mg / l NAA for 4 weeks