الفهرس 
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Abstract
A total of 150 samples of tilapia, mugel, tuna, saurus, pagrus and shrimp (25, of each) were randomly collected from different localities and fish shops at different sanitation levels in Zagazig city. The samples were taken under aseptic condition in sterile polyethylene bags and transferred in an ice box to the laboratory without delay. The samples were examined bacteriologically to isolate and identify Pseudomonas, Aeromonas and Vibrio species. In addition determine the antimicrobial susceptibility of the isolated microorganisms. Furthermore, PCR screen for virulence genes of A. hydrophila, V. parahaemolyticus, and V. cholera was determined.
The results displayed that 54 % of the examined fishes were contaminated with pseudomonas spp. The examined two types of fresh water fishes (tilapia and mugel) were positive for Pseudomonas 40 % and 56 %, respectively. The marine water fishes (tuna, saurus, pagrus, and shrimp) were positively Pseudomonas samples by 44 %, 60 %, 52 %, and 72 %, respectively.