الفهرس | Only 14 pages are availabe for public view |
Abstract Primary angle-closure glaucoma is a neurodegenerative disease characterized by elevated IOP due to a mechanical obstruction of the TM either by apposition of the peripheral iris to the TM or a synechia closed angle. PACG is a complex heterogeneous disease with multifactorial causes that can affect the progression of the anterior chamber angle from narrow to angle-closure. These factors include genetic factors and oxidative stress. The current study aimed to investigate the associations between PACG and rs11024102 in PLEKHA7, rs3753841 in COL11A1, and the systemic oxidative stress markers in Egyptian patients. This case-control study enrolled 35 control subjects and 64 PACG patients. The polymorphisms in PLEKHA7 and COL11A1 were analyzed using qRT-PCR followed by statistical analysis for their associations with PACG. In addition, the serum levels of MDA, AOPP, PC, and IMA (oxidative stress biomarkers) were quantitated colorimetrically. Finally, the associations of the oxidative stress biomarkers with both PACG and elevated IOP were statistically analyzed. The results of the current study showed that neither significant difference in the genotype distribution nor allele 87 frequency of PLEKHA7 11024102 T>C (p > 0.05) and COL11A1 rs3753841 G>A (p > 0.05) are recorded under any of the tested genetic models (homozygous, heterozygous, dominant, and recessive). Moreover, both rs11024102 PLEKHA7 and rs3753841 COL11A1 did not show a significant association with the increased risk of PACG (p > 0.025 after Bonferroni correction) in Egyptian patients. Patients with PACG showed significant elevations in the serum levels of MDA, AOPP, and PC either in patients with or without diabetes mellites, hypertension, CVD, and smoking. On the other hand, the serum level of IMA showed no statistically significant change (p > 0.05) in PACG patients. Of these oxidative stress biomarkers, MDA, AOPP, and PC were significantly associated with PACG in Egyptians (p < 0.013 after Bonferroni correction). Multiple linear regression analysis showed a significant correlation between the serum level of MDA and the elevation in the IOP in PACG patients (p = 0.005) (n=64) including cases with DM, hypertension, CVD, and smoking. Surprisingly, both MDA and PC levels showed significant correlations with the elevated IOP (p = 0.007 and 0.045, respectively) after excluding the cases of DM, hypertension, CVD, and smoking (n = 36). 88 Interestingly, an independent T-test performed to explore the effect of using the intraocular medications on the correlations of the oxidative stress biomarkers with the elevated IOP reports that the serum level of MDA showed a more elevation in PACG patients under intraocular medications (n=34) than patients who did not take any intraocular medications (n=2) (T = -2.33, p = 0.033). Moreover, the serum level of IMA showed a more reduction in patients under the intraocular medication (T = 2.91, p = 0.006). In conclusion, PLEKHA7 rs11024102 T>C and COL11A1 rs3753841 G>A SNPs could not be considered risk factors for PACG in Egyptians. However, systemic oxidative stress (the elevating serum levels of MDA, AOPP, and PC) was found to be a probable risk factor for PACG. Of these markers, only serum levels of MDA and PC were considered significant predictors for the elevation in the IOP average. |