الفهرس 
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Abstract
Background: The Most Prevalent Cancer In Egypt Is Hepatocellular Carcinoma (HCC) Mainly Due To The Infection With The Hepatitis C Virus. So It Is Critical To Find Sensitive Biomarkers For Early Diagnosis Of HCC And Avoid Post-Operation Tumor Recurrence. Therefore, This Research Was Designed To Demonstrate The Circserpina3 Role In The Regulation Of Microrna-944 Gene Expression In HCV-Related HCC Cases And Compare These Results With Circserpina3 And Microrna-944 Gene Expression Levels In HCV-Infected Patients.
Methodology: Study Participants Were Divided Into Three Groups: Healthy Controls, HCV-Infected, And HCV-Induced HCC Patients. The Gene Expression Levels Of Circserpina3 And Microrna-944 Were Evaluated Using Real-Time Qpcr. Then The Immunoblotting Procedure Was Applied To Measure The Protein Expression Levels Of Murine Double Minute 2 (MDM2) And E-Cadherin Besides, The Concentration Levels Of Glypican-3 And Alpha-Fetoprotein Were Measured By Sandwich ELISA.
Results: The Gene Expression Level Of Circserpina3 Was Significantly Upregulated In Both HCV-Infected And HCC Patients Causing Suppression Of The Antitumor Effect Of Mir-944 And Showing A Lower 1-Year Survival Rate Than The Participants Who Had Low Circserpina3 Gene Expression Level. Subsequently, The Mir-944 Downstream Protein, MDM2 Was Remarkably Upregulated, Exaggerating The Metastasis And Oxidative Stress In HCC Cases. Additionally, The Results Confirmed The Downregulation Of Microrna-944 Improved The Progression Of Viral Hepatitis C Cases To Hepatocarcinogenesis Through The Significant Increased Plasma Level Of Metastatic Marker, E-Cadherin. Although, Alpha-Fetoprotein Is A Common Diagnostic Marker Used In The Diagnosis Of HCC, Our Results Showed That Glypican-3 Had Greater Sensitivity And Specificity And Positively Correlated To The IGF-1 Signaling Pathway Of HCC Cases. Moreover, The Relative Expression Levels Of Circserpina3 And E-Cadherin In Both The HCV And HCV- Induced HCC Were Significantly Positively Correlated.
Conclusion: Circserpina3 And Mir-944 Were Sensitive Molecular Markers For Early Diagnosis Of HCC And Could Be Prospective Treatment Targets For HCV-Infected Patients To Avoid Tumor Recurrence In HCC Cases.
Keywords: Mir-944; Circserpina3; Glypican-3; Metastasis; Hepatitis C