الفهرس 
Introduction
PyroptosisOnly 14 pages are availabe for public view
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Abstract
GU is one of the most prevalent GIT diseases, that affects about 10% of world population. Treatment of this disease mainly depends on anti-secretory drugs such as PPIS and H2RAs, which have many adverse effects. This led to an attempt to find a treatment from a natural plant that is safe and has no side effects instead of these drugs. One of these most common medicinal plants is Aloe vera, which has powerful antioxidant, anti-inflammatory, and wound healing actions.
So, this study was designed to evaluate the potential therapeutic effect of Aloe vera gel in ethanol-induced GU in rats and to elucidate the underlying mechanisms involved. This through evaluating its effect on UI, I%, pyroptosis (NLRP3, GSDMD, and MYD88), gastric acid (gastrin and gastric pH), and oxidative stress (MDA and GSH). Pantoprazole was used as a reference drug.
The study was performed on 52 male albino rats distributed at random into four groups: control, ulcer, pantoprazole, and Aloe vera group. GU was induced via giving only a single oral dose of absolute ethanol (5ml/kg). Aloe vera (200 mg/kg/day) and pantoprazole (40mg/kg/day) treatment were given orally for consecutive14 days after ulcer induction.
At the end of the experiment, after rat sacrificing and stomach harvesting, gross evaluation of stomach, UI, I%, and gastric pH measurement were done. Gastric NLRP3, GSDMD, and MYD88 relative mRNA expression levels were estimated by qRT-PCR. Serum gastrin level was measured by ELISA. Gastric MDA and GSH were determined using spectrophotometric method. Finally, the histopathological examination was done using light microscopy.
The results of the present study showed the following:
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Ulcer group revealed a significant increase in UI, relative mRNA expression of NLRP3, GSDMD, and MYD88 in stomach, serum gastrin level, and gastric MDA, and a significant decrease in gastric pH and GSH when compared to control group. Gross investigations revealed a haemorrhagic ulcerative lesion in the stomach. Histopathological results showed severe epithelial loss, haemorrhage, oedema, inflammatory cells infiltration, and BVs congestion.
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Aloe vera treatment improved these gross, biochemical, molecular, and histopathological alterations induced by ethanol. It revealed a significant decrease in UI, relative expression of NLRP3, GSDMD, and MYD88 in the stomach, serum gastrin level, and gastric MDA, and a significant increase in gastric pH and GSH when compared to the ulcer group. It also provided a high I% of 91%.
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Pantoprazole treatment also improved the afore-mentioned alterations induced by ethanol. It revealed a significant decrease in UI, gastric relative expression of NLRP3, GSDMD, MYD88, and gastric MDA, and a significant increase in serum gastrin level, gastric pH, and gastric GSH when compared to the ulcer group. It also provided an I% of 76.17%.
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When reviewing the results of conventional treatment with pantoprazole and, in contrast, Aloe vera gel under study, we find that both have a significant effect on alleviating pyroptosis, gastric acid secretion, and oxidative stress, as well as reducing the histopathological changes. However, by comparing them, it was found that Aloe vera gel outperformed its pharmaceutical counterpart, pantoprazole, in improving all these biochemical markers measured in blood and tissue. It also outperformed pantoprazole in reducing histopathological changes, as illustrated in the accompanying tables and their statistical representation.