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العنوان
Biodiversity and identification of a DNA marker linked to bolting gene (s) in Egyptian sugar beet (Beta vulgaris L.) lines =
المؤلف
Farghaly, Asmaa Youssef Mekhemer,
هيئة الاعداد
باحث / اسماء يوسف مخيمر فرغلي
مشرف / محمد نجيب بركات
مشرف / سناء ابراهيم محمد ميلاد
مشرف / نبوية صالح عبدة غره
الموضوع
Crops.
تاريخ النشر
2018.
عدد الصفحات
83 p. :
اللغة
الإنجليزية
الدرجة
ماجستير
التخصص
العلوم الزراعية والبيولوجية
تاريخ الإجازة
13/12/2018
مكان الإجازة
اتحاد مكتبات الجامعات المصرية - Crops
الفهرس
Only 14 pages are availabe for public view

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Abstract

The present study has been carried out during the period 2013-2018. Five diploid multigern Egyptian sugar beet populations were used in this research. These populations were obtained from Sabahia Agriculture Research Station, Sugar Crop Research Institute, Agricultural Research Center-Ministry of Agriculture (Project of Breeding and Producing Sugar Beet Seeds under Egyptian Conditions). These populations named: EG26- 2014, EG27- 2014, and EG27-
2015 EG26- 2016 and EG27- 2016.
The study aims to accomplish the flowing objectives:
1. The assessment of genetic diversity were conducted using agro-morphological traits as well as ISSR and SSR markers and subsequently the genetic purity was assessed within the populations.
2. Identification of DNA markers linked to bolting gene (s) in Egyptian sugar beet lines using SSR technique.
Two experimental approaches were designed and applied in this study. The first approach represents the field trial experiment in Wade El-Natrona, El-Behera Governorate, Egypt. The second approach represents the Laboratory experiment, which was carried out in the
Biotechnology Laboratory, Crop Sciences Department, Faculty of Agriculture, Elshatby, Alexandria University, Egypt.
The present investigation was accordingly carried out through three main approaches as follows:
1. Assessment of the uniformity using agro- morphological traits a. Agro- morphological traits analysis
There were significant variations among sugar beet populations for the mean values of the number of leaves, leaf weight, leaf length, leaf width, petiole length, root weight, T.S.S%, root
length and root diameter. No significant differences were obtained in the number of leaves, leaf weight, leaf length, leaf width, petiole length, root weight, T.S.S%, root length and root diameter
within the plants of any of the two sugar beet genotypes. Cluster analysis and genetic similarity Summary and conclusion 69 matrix were carried out for all studied populations based on previously measured agromorphological traits. Results revealed that very high degree of similarity for almost all the plants within each of the populations EG26 and EG27 were obtained. However, a low genetic similarity or high genetic variation were obtained between the plants of the two populations EG27and
EG26. This means that, very high degree of similarity for almost all the vegetative characteristics
within each of the genotypes EG26 and EG27. Therefore, it could be clarified that within each of the two Egyptian genotypes EG26 and EG27, the sugar beet plants are genetically homogenous.
It could be concluded that each of these genotypes would be stable among years of cultivation and would be highly promised for commercial cultivation.