الفهرس 
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Abstract
Synanthropic filth flies are adapted to live in close proximity to human and animal habitations, more than any other group of insects; they have a major negative economic impact, significant veterinary and medical implications on people and animals. Cattle manure and animal barns are favorite breeding places and food sources for synanthropic flies. This strong attraction makes various species of flies efficient transmitters of many pathogens therefore this study was done to identify various species of synanthropic filth flies in three animal rearing stations in Assiut Governorate, Upper Egypt and the seasonal variation affecting the abundance of these flies in the selected study sites. Furthermore we aim to diagnose possible parasitic infestation on the collected flies to confirm the role of synanthropic flies as mechanical vectors for parasitic infection using morphological and molecular tools.
In the present study, variations in temperature and relative humidity throughout the study period from July 2020 to June 2021 were recorded. The mean morning temperature in the three animal stations during one-year interval ranged between 17.00°C to 32°C±0.5 °C. While, the average late temperature ranged between 32.5°C to 40.5°C±0.5 °C. The highest recorded temperatures during summer (June to August), with relative humidity ranged between 20.5% to 36.3%±0.5 % while the lowest recorded temperatures during winter (December and January), with relative humidity ranged between 52.75% to 55.3%±0.96%.
• Seasonal abundance of dipteran families and species in the three animal rearing stations
The abundance of the collected synanthropic filth flies was studied in three animal rearing stations in Assiut Governorate from July 2020 to June 2021. A total of 12749 flies were collected from the selected study sites. The collected fly species belonged to seven dipteran families; Muscidae (7978), Sphaeroceridae (2910), Fannidae (1134), Ulidiidae (137), Sepsidae (581), Calliphoridae (7), and Sarcophagidae (2).
Family Muscidae was the most prevalent family in all stations (62.58%) followed by families Sphaeroceridae (22.83), Fannidae (8.89), Sepsidae (4.56) and finally Ulidiidae (1.07).
Families Calliphoridae (0.055) and Sarcophagidae (0.02) were the least presented families in all collection sites. Also, Family Sarcophagidae wasn’t detected in Station (C) during the study.
The highest numbers of flies were collected from animal rearing station (B) (5890, 46.20%) followed by the station (A) (4323, 33.91 %) and the station (C) (2536, 19.89 %), respectively.
The seasonal abundance of family Muscidae in the three animal stations
Family Muscidae was the most prevalent synanthropic fly family all over the seasons in the three collection sites.
M. domestica and Stomoxys calcitrans were collected in all seasons of the study. M. sorbens was collected only in summer and autumn. Out of Muscidae species, Musca domestica was the most prevalent species. Its highest abundance was in summer followed by spring and autumn, respectively whereas winter showed the least fly abundance. Regarding the collection sites, station (B) showed the highest abundance in M. domestica, compared with other stations, in summer, autumn, and spring with statistically significant difference (P <0.001). However, animal station (A) showed high prevalence in M. domestica in spring (P <0.001).
Regarding to S. calcitrans, station (C) was the most common location for this species. The highest percentage of this species were reported in summer followed by spring, and autumn with statistically significant difference (P <0.001). Musca sorbens showed the least presentation of this family found in station (A) during the summer and autumn.