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العنوان
Studies On Utilization Of Enzymes In Functional Foods/
المؤلف
El-Sayed, Amr Ahmed Ahmed.
هيئة الاعداد
مشرف / Amr Ahmed Ahmed El-Sayed
مشرف / Samy Mohamed Abu El-Maaty
مشرف / Magdy Mohammed Abdelhady
مشرف / Hammam El-Tukhy Mohamed Bahlol
الموضوع
Food Science.
تاريخ النشر
2023.
عدد الصفحات
143 P. ;
اللغة
الإنجليزية
الدرجة
الدكتوراه
التخصص
علوم وتكنولوجيا الأغذية
تاريخ الإجازة
14/6/2023
مكان الإجازة
جامعة الزقازيق - كـليـــة الزراعـــة - علوم الأغذية
الفهرس
Only 14 pages are availabe for public view

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from 165

Abstract

Since its discovery in many heat-treatment foods in 2002, many efforts have been made to reduce acrylamide levels in foods. Methods to reduce acrylamide levels by reducing Maillard reaction products have been considered. However, baking cookies and the processing of potato chips, induce acrylamide, a carcinogenic compound, during the manufacturing. This study aimed to use a new quantitative index and formula from L-asparaginase, glucose oxidase, their 1:1 blending enzymes, baker’s yeast, and green tea powder (0.5 g/kg wheat flour) at a new proposed temperature of 37 oC for 30 min to reduce acrylamide production in biscuits and bakery products using new indicators such as asparagine reduction (%), the asparagine/acrylamide ratio, acrylamide reduction (%), and the asparagine/reducing sugar ratio. The highest acrylamide concentrations were reduced from 865 mg/kg in the blank sample (BT0) to 260 and 215 mg/kg in the mixed enzyme powder (1:1) (BT3), and BT4-treated samples, respectively. The biscuit samples treated with 0.5 g/kg L-asparaginase reduced the acrylamide levels by approximately 67.63%, while the BT3 samples showed acrylamide levels of 69.94% and asparagine levels of 68.75% and 47%, respectively, compared with percentage in the untreated sample (blank), 95%. This percentage was 54.16% for the BT4 samples. This study also aimed to use a new quantitative index and formula for L-asparaginase, glucose oxidase, their 1:1 blending enzymes, baker’s yeast and green tea powder (0.5 g/kg distilled water) to reduce acrylamide production in potato chips and evaluate the products on some new indicators, such as asparagine reduction (%), acrylamide reduction (%), Asparagine / Acrylamide ratio (AAR) and Asparagine content (mg/kg). The highest acrylamide concentrations were reduced from 1346.0±7.9 mg/kg in the blank sample (control) to 285.7±3.2 and 325.3±10.5 mg/kg in the baker’s yeast sample (CT4) and green tea sample (CT5) treated sample, respectively. The potato chips treated with 0.5 g/kg L-asparaginase (CT1) reduced the acrylamide level by approximately 70.65±0.31%, while the CT4 samples showed 78.77±0.21% and asparagine contents of 116.0±5.3 mg/kg and 195.0±5.0 mg/kg, respectively.The results showed that acrylamide was formed during baking and frying processes, and all treatment samples inhibited its formation, making it possible to produce foods with low levels of acrylamide in starchy foods in the food industry sector at 37 oC for 30 min and preserving the quality and nutritional value of the final product. It can be used as a specialty food or functional food and protects school-age children, as well as youth on campus, from approximately 70–80% of their daily intake of acrylamide.Keywords: Acrylamide; Asparagine; Baker’s yeast, Biscuits; Glucose oxidase; L-asparaginase; Maillard reaction; Potato chips.