الفهرس 
Introduction and Aims of the studyOnly 14 pages are availabe for public view
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Abstract
The first objective of this research is to test the validity of the bio-resistance of Pseudomonas bacteria to plant fungi. Therefore, four sp. of bacteria were used, and in an experiment on petri dishes, the ability of the four types of bacteria, namely
P.Fluorescense, P.Putida, P.Mendocina, and P.Aeruginosa, was proven to be resistant to fungi F.Solani, F.Oxysporum, and R.Solani
The second goal of the work is to use DNA barcoding and the rpoD, rRNA (16S)
primers to distinguish between different species of pseudomonas bacteria at the
molecular level. Pseudomonas samples from every collection were sequenced; the sequences were given accession numbers and assigned to the National Center for Biotechnology Information (NCBI). Also, using BLAST, the nucleotide sequences of the (16S) genes from Pseudomonas sp. samples collected were aligned with other rbcL
genes from the GenBank database (http://www.ncbi.nlm.nih.gov)
(https://blast.ncbi.nlm.nih.gov/Blast.cgi?PROGRAM=blastn&PAGE_TYPE=BlastSea
rch&LINK_LOC=blasthome). Using the MEGA11 software programme, a
phylogenetic tree was created for the 16S genes between the investigated samples, which came from department of microbiology, Ain shams University, Egypt, and others that were identified in GenBank and had the capacity to biocontrol fungi.
The results of this work are as follows:
1- Bio-control:
Pseudomonas bacteria were utilised as a clean and ecologically acceptable
biological control agent to develop fungicide resistance without the emergence of any
Summary
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environmental contamination or adverse impacts on the economic plants. The current analysis established evidence for these bacteria’s resistance to fungus and the occurrence
of an inhibitory zone. On efficient defence.