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العنوان
DNA barcoding of some Pseudomonas species as biocontrol agents /
المؤلف
shaaban, Ebrahim Salem Ebrahim.
هيئة الاعداد
باحث / EBRAHIM SALEM EBRAHIM SHAAPAN
مشرف / Mohammed M. Abdel Fatah Yacout
مشرف / Huda Ebrahim Shakkam
مشرف / Moustafa Mahmoud Eldakak
تاريخ النشر
2023.
عدد الصفحات
98 p. :
اللغة
الإنجليزية
الدرجة
ماجستير
التخصص
العلوم الزراعية والبيولوجية
تاريخ الإجازة
20/10/2023
مكان الإجازة
اتحاد مكتبات الجامعات المصرية - وراثة
الفهرس
Only 14 pages are availabe for public view

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Abstract

The first objective of this research is to test the validity of the bio-resistance of Pseudomonas bacteria to plant fungi. Therefore, four sp. of bacteria were used, and in an experiment on petri dishes, the ability of the four types of bacteria, namely
P.Fluorescense, P.Putida, P.Mendocina, and P.Aeruginosa, was proven to be resistant to fungi F.Solani, F.Oxysporum, and R.Solani
The second goal of the work is to use DNA barcoding and the rpoD, rRNA (16S)
primers to distinguish between different species of pseudomonas bacteria at the
molecular level. Pseudomonas samples from every collection were sequenced; the sequences were given accession numbers and assigned to the National Center for Biotechnology Information (NCBI). Also, using BLAST, the nucleotide sequences of the (16S) genes from Pseudomonas sp. samples collected were aligned with other rbcL
genes from the GenBank database (http://www.ncbi.nlm.nih.gov)
(https://blast.ncbi.nlm.nih.gov/Blast.cgi?PROGRAM=blastn&PAGE_TYPE=BlastSea
rch&LINK_LOC=blasthome). Using the MEGA11 software programme, a
phylogenetic tree was created for the 16S genes between the investigated samples, which came from department of microbiology, Ain shams University, Egypt, and others that were identified in GenBank and had the capacity to biocontrol fungi.
The results of this work are as follows:
1- Bio-control:
Pseudomonas bacteria were utilised as a clean and ecologically acceptable
biological control agent to develop fungicide resistance without the emergence of any
Summary
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environmental contamination or adverse impacts on the economic plants. The current analysis established evidence for these bacteria’s resistance to fungus and the occurrence
of an inhibitory zone. On efficient defence.