الفهرس 
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Abstract
This hospital based descriptive cross-sectional study included 261 patients who were admitted to clinical hematology department at Assiut University Hospitals during the period from January 2020 to February 2022.
This study was trying to identify bacterial and fungal species causing bloodstream infection (BSI) in hematological malignancies, the incidence of bacterial and fungal BSIs in study group inside our institute, identify antibiotic sensitivity pattern and antifungal sensitivity pattern for causative microbes, and compare culture on ordinary media with Vitek2 (automated microbial identification system) and multiplex PCR (Biofire filmarray BCID Panel).
All study group patients were subjected to full history taking including age, sex, type of malignancy, state of disease, line of administration (central or peripheral) and antibiotic or antifungal prophylactic protocol, clinical examination, routine and microbiological laboratory investigations which were performed in Microbiology unit, Clinical Pathology Department at Assiut University Hospital.
Microbiological diagnosis includes blood cultures which were done according to SOPs of microbiology laboratory. Blood cultures were inoculated into BacT/ALERT culture bottles then incubated in the BACT/ ALERT 3D system (BioMerieux, France). Identification of isolated organism were done according to manufacturer instructions by VITEK 2 Compact, Biofire filmarray BCID Panel and conventional manual identification.
The result revealed that the incidence of BSIs among studied population was 20.7% of total 261 cases. Gram negative bacteria were the main responsible for the BSIs in our study patients, which account 57.6% while Gram-positive bacteria accounted for 35.2% and fungi account for 7.4%.
Our study data show that both pathogen type and pattern of antibiotic resistance can affect outcomes of hematological malignancies patients as more non-survivors found among gram negative BSIs and specifically among Carbapenemase resistance gram negative isolates.
In this study, the results of the Biofire FilmArray BCID panel in comparison to VITEK2 and conventional identification of pathogen in positive blood culture bottles show sensitivity, specificity of 100.0% in diagnosis of gram positive organism, gram negative organism (E.Coli, Klebsiella pneumonia, Pseudomonas aeruginosa) and fungi (candida albicans).