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Abstract This work was carried out at the Genetics Department, Faculty of Agriculture, Ain Shams University. The aim of this work was to Screen and isolate the soil fungal community from rhizosphere and non-rhizosphere soil from newly reclaimed lands in comparison with uncultivated ones (desert). In addition to identifying the obtained fungal spices using ITS rDNA sequencing. followed by Investigating the effects of soil physicochemical properties on the fungal communities on the structure, composition, and metabolic profiles, and determining the strains with the potential ability to solubilize phosphor and produce indole acidic acid (IAA) in vitro. To produce a baseline for the future development of fungi based on biofertilizers, and biocontrol plans associated with land reclamation. The research process was as follows: 1- Performing soil physicochemical analysis to determine sample elements ratios ex; Soil pH, moisture content (MC), total carbon (TC), total nitrogen (TN), organic carbon (SOC), organic carbon (TOC), Soil microbial biomass carbon (MBC) and nitrogen (MBN). Isolation and enumeration of most representative fungal colonies from the collected soil samples, using final culture media (DRBC, and PDA). 2- Extract the genomic DNA from each isolated fungal strain, perform molecular identification using the ITS rDNA gene which was amplified using a universal primer pair (ITS1 and ITS4), and PCR products were sequenced. 3- Perform a phylogenetic analysis of the obtained sequences to determine their taxonomical position, followed by registration in the gene bank database. 54 SUMMARY Mostafa N. M. Ali (2023), M. Sc., ALARI., Ain Shams Univ. 4- Testing the obtained fungal strains for their ability to solubilize phosphor and produce IAA in vitro. The obtained results were as follows: Among the screened soil samples 272 fungal strain was counted and isolated, yielding 27 filamentous fungal species belonging to phyla Ascomycota, from eight genera. Most of the fungal isolates were identified at the species level as Aspergillus, Penicillium, Epicoccum, Fusarium, Ulocladium, Trichoderma, Cladosporium, and Chaetomium. The 27 fungal strains were subjected to test their ability for P solubilization, and IAA production with tryptophan in different concentrations at different pH levels.12 strains were able to produce different sizes of halo p zone around the colony growth on PVK media, while 10 species resulted positive and varied in their ability to produce IAA which proved their potential ability to be used as a microbe-based phosphatic fertilizer, and plant-promoting growth (IAA) Eco-friendly biofertilizers. The findings of the current study can serve as a baseline for the future development of fungal-based antagonistic on soil microbiome, plant growth, and biocontrol research. It can provide an outline of the pathogenic fungal populations associated with the vegetation type of the studied orchards (i.e., Citrus and Olives). Meanwhile the obtained data could suggest that colonization of fungal in the rhizosphere, and nonrhizosphere of the studied locations is independent of the P-solubilizing activity and IAA production, which could have a prospective effect on the growth and yield of the plantation, provide potential candidates as biofertilizers |