الفهرس | Only 14 pages are availabe for public view |
Abstract In rabbit farms, artificial insemination is usually undertaken using preserved semen. However, increased reactive oxygen species levels during preservation produce sperm dysfunction. Mammalian spermatozoa are highly susceptible to reactive oxygen species (ROS) stress. This study assessed the influence of supplementing the rabbit semen extender with various concentrations of glutathione (GSH) and taurine at 24, 48, and 72 h postchilling at 5°C. Semen samples were collected from 20 New Zealand bucks, and ejaculates with standard color, motility (>85%), about 0.5 mL volume, and ∼400 ×106 /mL concentration were used and diluted with extenders supplemented with 0.5, 1, and 2 mM of GSH and 1, 5, and 10 mM of taurine and chilled at 5°C. Nonsupplemented samples were used as a control. Sperm’s progressive motility, acrosome reaction, and extracellular oxidative stress biomarkers such as MDA contents and SOD, CAT, and GPx concentrations, total cholesterol, triglyceride, total protein, GPT, fructose and intracellular transcriptomic levels of SOD and CAT genes were assessed. Results showed that, in general, glutathione and taurine supplementation significantly improved sperm motility and viability percent compared to control after 24, 84 and 72h post chilled storage. The supplementations improved the sperms kinetics by reducing cooling-associated stress, which was ascertained by lowering MDA concentration, preserving membrane stability through obvious decreasing of GPT, chol, triglyceride, total protein at seminal level which implies sperm membrane stability and increasing SOD, CAT, and GPx activities . Increasing the levels of antioxidant enzymes in the extender was due to the increasing mRNA copies of the SOD and CAT genes. Furthermore,maintaining the fructose levels in the extender. In conclusion, GSH and taurine supplementation to the extender had protective influences on the in vitro rabbit semen quality during chilled storage for up to72 h at 5°C. |