الفهرس 
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Abstract
Brucellosis is a highly contagious bacterial disease that affects a wide range of animals (cattle, camels, sheep, goats, equines, and pigs) and humans, caused by Brucella species, it is considered one of the most economically significant endemic infectious diseases in Egypt, not only in animals but also in humans.
The present study was piloted for a period of one year and 2 months, extending from March 2022 to May 2023, and aimed to determine the seroprevalence, isolation, identification of Brucella spp. and studying risk factor associated with Brucellosis among animal species, in New Valley governorate.
RBPT was used as serological screening test which carried out on serum samples of 954 examined animals (250 cattle, 100 camels, 335 sheep, and 269 goats) of different ages and sexes from different localities.
Regarding to results of seroprevalence study by RBPT in cattle, camels, and sheep, and goats, results revealed that 20 cattle out of 250 examined cattle (8%), 12 camels out of 100 examined cattle (12%), 49 sheep out of 335 examined sheep (14.6%), and 45 goats out of 269examined goats (16.7%) were detected as seropositive.
In studying risk factors associated with Brucellosis, according to seasons, the highest seroprevalence among animal species by RBPT was (42.6%) in summer, followed by (11.4%) in spring, then (10.9%) in winter, and the lowest rate was (9%) in autumn while for locality, the highest prevalence of infection by RBPT was (22.3%) in EL-Dakhla, followed by (18.2%) in El-Kharga and (12.7%) in El-Farfra, then (9.8%) in Balat, and the lowest rate was (1.03%) in Paris.
Regarding to age, the highest prevalence of cattle infection was in aged (≤3 years) and the lowest rate was in cattle aged (<3- 6 years) , in camels, the highest prevalence of camel infection was in camel aged (<3- 6 years) and the lowest rate was in camel aged (≤3years), in sheep, the highest prevalence of infection was in aged (>2 years) and the lowest rate was in aged (>1 up to 2 years), and in goats the highest prevalence of infection was in aged (≤1years) and the lowest rate was in aged (>1 up to 2 years), and the prevalence in females (13.5%) was the highest infection rate compared to males (11.7%).
The CFT, and IELISA tests was used as a confirmatory test for RBPT, in RBPT positive samples (126), 37 samples (29.4%) were positive by CFT, while in 16 samples negative with RBPT, one sample (6.3%) was positive by CFT. While indirect ELISA test revealed that 126 serum samples positive by RPBT, 77 serum samples were positive by indirect ELISA test, while 16 serum samples were negative by the RBPT test, 5 positive samples by indirect ELISA test.
Isolation and identification of Brucella species, revealed seven Brucella field isolates had recovered from 95 lymph nodes of cattle from abattoir and two Brucella isolates from spleen of 5 aborted fetus of aborted cases, the isolate, were identified by multiplex PCR (Bruce – ladder) test, using specific primaries’, shown that, 5 isolates Brucella melitensis and 2 isolates of Brucella abortus isolated from L.Ns while 2 isolates of Brucella melitensis isolated from aborted fetuses.
In conclusion
• High seroprevalence level of infection with Brucellosis in cattle, camels, sheep, and goats in the five centers in New valley governorate.
• They are many risk factors associated with Brucellosis in animals including, species, age, locality, sex, and season.
• ELISA and CFT are two confirmatory tests, and CFT is most accurate conformity test used in diagnosis of Brucellosis.
• B. melitensis and B.abortus were the isolates identified by multiplex PCR among animals in New Valley governorate.