الفهرس 
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Abstract
To potentiate the anti-cancer properties of resveratrol (RES) via improving its
drawbacks by formulating it into a smart lipid.
RES-loaded Smart Lipid was prepared using a 21x32 factorial design, resulting in 19
formulae with three factors at different levels: (1) the total lipid concentration; (2) the
concentration of surfactant; and (3) the type of surfactant. High-shear hot
homogenization was used as a preparation method. Entrapment efficiency percentages
and particle size were chosen as the responses. Based on the result of the evaluation, the
five highest desirability preparations were selected for the in vitro release study.
Preparation with the best release pattern and storage condition was only selected for
characterization as transmission electron microscopy, Differential Scanning
Calorimetry, and Fourier Transform Infrared spectroscopy. Different cell lines were
used to compare the anti-cancer properties of the optimized preparation and the
cytotoxicity of RES alone.
The optimized preparation showed small particle size (288.63 ± 5.55 nm), zeta potential
(-16.44 ± 0.99 mV), and entrapment efficiency (86.346 ± 3.61 %) with spherical
morphology by transmission electron microscopy, Differential Scanning Calorimetry
showed no interaction between drug and other components, and the structure of the
compounds was confirmed by Fourier Transform Infrared spectroscopy. Finally, the
cytotoxic activity of the optimized RES-loaded Smart Lipid on different cell lines was
assessed through MTT assay, and its effect on cell cycle progression and apoptosis
induction were assessed, using flow cytometry and annexin V kit respectively. Our
results showed that RES-loaded Smart Lipid significantly reduced cell viability,
induced cell cycle arrest at G0/G1 phase and apoptosis compared to free formula and
free RES suspension.
Loading RES into this novel kind of nanocarrier enhances it is cytotoxicity properties.