الفهرس 
Introduction and Aim of the workOnly 14 pages are availabe for public view
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Abstract
Acute lung injury (ALI) is a significant medical condition characterised by the compromise of pulmonary function, ultimately resulting in respiratory failure and subsequent fatality. The induction of Acute Lung Injury (ALI) can be facilitated by both direct and indirect mechanisms. Lipopolysaccharide (LPS) serves as the principal determinant accountable for the toxicity linked to gram-negative bacteria and is frequently utilised in the development of acute lung injury (ALI) models. The objective of this study is to investigate the effects of a specific intervention on the target population The main aim of this study was to investigate the acute lung injury induced by lipopolysaccharide (LPS) and evaluate the potential protective effects of quercetin and vitamin D on these pathological changes. This section provides a description of the experimental procedures and materials utilised in the investigation. The rats were divided into four groups of equal size using a random allocation procedure. The experimental investigation comprised four distinct groups, namely a control group, an LPS-treated group, a group treated with Quercetin+Lipopolysaccharide (QUE+LPS), and a group treated with Vitamin+Lipopolysaccharide (Vit-D+LPS). In the group that received LPS treatment, rats were subjected to a solitary intra-tracheal administration of lipopolysaccharide (3mg/kg) on the 8th day of the experiment. Subsequently, the rats were euthanized 24 hours following the administration of LPS. The experimental group that received treatment with QUE+LPS was administered an oral dosage of QUE at a concentration of 100 mg/kg for a duration of 7 days. On the 8th day, a single intra-tracheal dose of LPS at a concentration of 3 mg/kg was administered. The animals in this group were then slaughtered 24 hours following the LPS injection. In a similar manner, the group that was treated with Vit-D+LPS was administered an oral dosage of Vitamin D (25mg/kg) for a duration of 7 days. On the 8th day, this group received a single intra-tracheal dose of lipopolysaccharide (LPS) at a concentration of 3mg/kg. The animals in this group were then sacrificed 24 hours following the administration of LPS. The main findings are as follows: The pre-treatment administration of quercetin and vitamin D exhibited protective benefits on pulmonary function, as indicated by a decrease in the concentrations of inflammatory biomarkers. The biomarkers examined in this study encompassed pulmonary Tumour Necrosis Factor-Alpha, broncho-alveolar surfactant– protein D, serum Ferritin, serum D-dimer, and pulmonary Advanced Glycosylation End Product Specific Receptor. Furthermore, there was a notable elevation observed in the prothrombin time level. In addition, this preventive measure was accompanied by the restoration of the equilibrium between oxidative stress and antioxidants, as indicated by the decrease in levels of pulmonary malondialdehyde and broncho-alveolar 8-Hydroxydeoxyguanosine. Furthermore, the presence of diminished caspase-3 immuno-reactivity was identified, providing additional evidence in favour of the protective effect. The aforementioned results were additionally validated through the observed enhancement in lung histopathology in comparison to the group subjected to LPS treatment. The findings of the present study revealed that Quercetin exhibited a stronger protective effect than vitamin D. This difference can be ascribed to the significant antioxidant, anti-inflammatory, and anti-apoptotic activities of both compounds, which ultimately led to a more pronounced improvement in lung histological features.
In conclusion, the results indicate that Quercetin exhibits promising therapeutic and preventative characteristics in relation to pulmonary illnesses. Furthermore, it is advised to administer vitamin D as a preventive measure, either alone or in combination with other pharmacological interventions, to decrease the potential for pulmonary damage.
The primary objective of this study is to examine the possible therapeutic benefits of lipopolysaccharide (LPS), quercetin, and vitamin D in the context of acute lung injury (ALI).