الفهرس 
AIM OF STUDYOnly 14 pages are availabe for public view
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Abstract
The present study was conducted with the aim of exploring some aspects related to the potential hepatoprotective activity of Delonix regia seeds powder (DRSP) against carbon tetrachloride intoxication in rat liver. Also, to explain the previous possible roles, the proximate composition, bioactive compounds content and antioxidant activity of these seeds powder will be in the scope of this investigation.
Experimental design
All biological experiments were performed in a complying with the rulings of the Institute of Laboratory Animal Resources, Commission on life Sciences . Rats (n=36 rats) were housed individually in wire cages in a room maintained at 25±2 0C, relative humidity (55±3%), a 12-hr lighting cycle and kept under normal healthy conditions. All rats were fed on basal diet (BD) for two-week before starting the experiment for acclimation. After two week period, the rats were divided into main groups. First group (6 rats), as a negative control group, fed on BD and injected with olive oil (5 ml/kg body weight) which was used as a vehicle for the treatment of animals in CCl4 group. Second main group (30 rats) was injected with CCl4 to induce liver impaired rats then classified into sex equal sub groups as follow: group (2), as a positive control group, fed on BD and groups (3-6) fed on BD containing 5, 10, 15 and 20% (w/w) Delonix regia seed powder (DRSP), respectively.
Results
Proximate composition, minerals, antinutritional compounds content and antioxidant activity of Delonix regia seed powder (DRSP)
The largest compound (50.49 ± 0.91 g.100g-1) followed by crude fat (16.91 ± 1.06 g.100g-1), crude fiber (13.73 ± 3.27 g.100g-1), total protein (9.04 ± 0.43 g.100g-1) and ash (1.25 ± 0.19 g.100g-1).
DRSP is rich in different estimated elements. The trend of the abundance of mineral concentration in the DRSP in decreasing order is as follows Na> Zn> Fe> Mn>Cu>K>Mg>P>Ca>Se.
Polysaccharides were the largest compound followed by polyphenols, saponin, oxalate, tannin, carotenoids, and kaempherol.
DRSP recorded the antioxidant activity equal 62.56 ± 1.19% which compares well with the references/standards antioxidants used i.e. BHT (50 mg/ml), BHT (100 mg/ml) and α-tocopherol (50 mg/ml) by 70.73 ± 1.37, 65.81 ± 1.66 and 65.06 ± 1.03%, respectively.
Biological studies of of Delonix regia seed powder (DRSP)
Effect of Delonix regia seed powder (DRSP) on body weight gain (BWG), feed intake (FI) and feed efficiency ratio (FER) of hepatic rats
At the end of the experiment (8 weeks), the CCl4-treated rats exhibited significantly (p≤0.05) decreased in BWG (-48.35%), FI (-34.31%) and FER (-32.88) compared to the normal group. However, intervention with DRSP (5, 10, 15 and 20 g/100g diet) in feeding rats for 4 weeks led to significantly (p≤0.05) decrease on the BWG, FI and FER of the hepatoytoxic rats (model control) by the rates of -46.15, -24.18, -14.29 and -8.79%, -32.1, -24.37, -17.00 and -9.85%, and -27.4, -15.1, -10.9 and -8.22% of the normal group, respectively. The rate of decreasing in BWG and FI of the hepatotoxic rats were exhibited a dose- dependent manner.
Effect of Delonix regia seed powder (DRSP) on organs weight (g) of hepatic rats
Injection of rats with CCl4 (model control) leads to decrease the liver, spleen and kidney weight than the normal group by the rate of -29, -12.5 and -14%, respectively. However, intervention with DRSP (5, 10, 15 and 20 g/100g diet) in feeding rats for 4 weeks led to significantly (p≤0.05) increase on the liver, spleen and kidney weight of the hepatoytoxic rats (model control) by the rates of -10.8, 4.6, -3.0 and 4.6%, -8.10, -6.25, -2.50 and 0.0%, and -5.71, -2.14, -1.43 and -1.43% of the normal group, respectively. The rate of increasing in organs weight of the hepatotoxic rats was exhibited a partially dose- dependent manner.
Effect of Delonix regia seed powder (DRSP) on liver functions of hepatic rats
At the end of the experiment (8 weeks), the CCl4-treated rats exhibited significantly (p≤0.05) increased in AST (115.68%), ALT (120.70%) and ALP (85.2%) compared to the normal group. However, intervention with DRSP (5, 10, 15 and 20 g/100g diet) in feeding rats for 4 weeks led to significantly (p≤0.05) decrease on the AST, ALT and ALP by 101.79, 89.58, 56.00 and 33.00%, 105.70, 84.20, 53.20 and 26.50%, and 36.97, 28.16, 21.10 and 13.53% of the normal group, respectively. The rate of decreasing in AST, ALT and ALP of the hepatotoxic rats were exhibited a dose- dependent manner.
Effect of DRSP on serum albumin and total protein of hepatic rats
At the end of the experiment (8 weeks), the CCl4-treated rats exhibited significantly (p≤0.05) decreased in Alb (-30.9%) and TP (27.0%) compared to the normal group. However, intervention with DRSP (5, 10, 15 and 20 g/100g diet) in feeding rats for 4 weeks led to significantly (p≤0.05) decrease on the Alb and TP by-23.8, -21.4, -14.0 and -7.0%, and -23.0, -13.6, -10.9 and -6.8% of the normal group, respectively. The rate of decreasing in Alb and TP of the hepatotoxic rats were exhibited a dose- dependent manner. In similar studies, CCl4-induced significant decrease in the serum albumin and total protein content as the consequence of liver injury . Indicated that hypoalbuminaemia is most commonly in the case of advanced chronic liver diseases and a useful index of the severity of cellular dysfunction in such diseases. Data of the present study indicated that DRSP significantly (p≤0.05) increased serum Alb and TP levels which demonstrating that it can prevent or repair the liver cells damage. Such a role of the DRSP in treating the serum Alb level is of high importance, since human serum Alb is the major protein of blood plasma i.e. constitutes about 50%. In this way, Alb is the transport protein that binds to and carries around various ligands such as water, fatty acids, hormones, bilirubin, thyroxin hormone, pharmaceuticals, and cations. .