الفهرس | Only 14 pages are availabe for public view |
Abstract Studying the effect of some natural products against Helicobacter pylori Helicobacter pylori bacteria has become more common as a major cause of gastrointestinal diseases, and more than 60% of the world’s adult population is infected with it, as it is the main cause of stomach ulcersthat may lead to stomach cancer. The goal of the work: Use some natural products to eliminate Helicobacter pylori bacteria. The results we obtained can be summarized as follows: - Helicobacter pylori bacteria were isolated from biopsy samples of infected patients. - The best conditions for its growth were to use Columbia blood agar, in addition to the skirrow supplement, which stimulates the growth of these bacteria more than others and inhibits others, by using carbon dioxide incubation at (CO₂ 10%, O₂ 5%, N₂ 85%) 37and pH 7.3 for three days. - Biochemical tests were performed and were found to be positivefor catalase, oxidase, and urease, in addition to the Gram stain, and were found to be Gram negative and appear curved bacilli shape. - An identification was made using 16SrRNA and it was taken as no on the gene bank (OK 298394). - Natural extracts were prepared and tests were conducted to determine their effect on inhibiting the isolated bacteria, and the extract that had the highest effect against bacteria was identified, which is tamarind. SUMMARY - Concentrations were made to determine the maximum effect of different concentrations. - This tamarind extract was used to synthesize selenium nanoparticles and photograph these molecules using an electron microscope (TEM) and perform FTIR analysis and UV analysis. - These molecules were separated and their effect on the isolated Helicobacter pylori bacteria and other standard strains was observed. This effect was photographed using an electron microscope (SEM), and cell wall damage and shrinkage in size compared to the control was observed. |