الفهرس 
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Abstract
This study was planned to detect the prevalence of Aeromonas hydrophila in
some dairy farms where that 22 milk samples of cattle origin out of 380
examined cattle milk samples were positive for A. hydrophila with prevalence of
5.8% (n=22). The bacteriological examination of The environmental samples was
carried out for detection of A. hydrophila , 10 milk tank samples, 10 water
samples , 150 bedding samples and 10 animal feed samples and the results
showed that, the prevalence of A. hydrophila in environment, the positive
samples were 2 milk tank samples (20%), 2 water samples (20%), 11 isolates
from bedding samples (7.3%) and no isolates from feed samples ( 0%). All
isolates were confirmed as A. hydrophila by studying the morphological
characters on the specific culture media as well as its characteristic biochemical
reactions. The molecular study on the A. hydrophila isolates showed that 22
strains of A.hydrophilia, 14 strains (63.6%) carry no virulence genes and 8 strains
(36.4%) carry aerA gene, 5 of them (22.7%) carry hlyA gene also, while 3 strains
(13.6%) carry aerA gene only. Antibiotic sensitivity test showed that the results
recorded the highest resistance of A. hydrophila to ampicillin and lincomycin
(100%) followed by cefotaxime (90.9%). None of the tested 22 isolates was
resistant to amikacin. The public health importance was studied especially with
the results of the contaminated milk samples and the environmental examined
samples.