الفهرس 
Alzheimer’s disease (AD)Only 14 pages are availabe for public view
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Abstract
1-Lyophilized primer at -20ºC was equilibrated at room temperature.2- The equilibrated primer was spin down for 3 sec using spin-centrifuge-vortex. 3- The lyophilized primer was diluted (both forward and reverse) with RNase free water (the volume was added to get 100μM stock) and then the tube was gently inverted for 2 min at room temperature. 4- The stock primer was diluted with RNase free water buffer (pH 8.0) to get 5μM and kept at -20ºC until used (Monis et al., 2006).