الفهرس 
Heart failureOnly 14 pages are availabe for public view
 |  | from | 148 |  |  |
| | | from | 148 | | |
Abstract
Heart failure is a chronic disease, which poses a growing problem for
health systems worldwide as morbidity and mortality are increasing
annually. HF can appear in the presence of reduced ejection fraction
(HFrEF), mild reduced ejection fraction (HFmrEF) and preserved ejection
fraction (HFpEF).
The N-terminal pro-hormone brain natriuretic peptide (pro BNP) is
secreted into the blood stream by cardiac myocytes in response to increased
ventricular wall stress, it was regarded as a biomarker with potential role in
distinguishing HF. Over the last decade, it has been found that circulating
microRNAs are involved in the pathogenesis of cardiovascular diseases.
MicroRNAs are endogenous, non-coding, single-stranded RNAs consisting
of fewer than 22 nucleotides.
It was reported that microRNA-19b, microRNA-21and microRNA
208a are involved in cardiovascular biology and disease and have received
significant attention in the heart disease diagnosis. Identification of
specific, reliable and sensitive biomarkers for HF is a field of active
cardiovascular research, so the present study aimed to explore the
association between the levels of circulating microRNAs 19b, 21, 208a
and pro BNP with HF.
The study included 45 cases of confirmed HF in addition 15 controls
and categorized into 4 groups:
1. group I: Heart failure patients with preserved ejection fraction
(HFpEF) assessed by echocardiography ≥ 50%.
2. group II: Heart failure patients with mild reduced ejection fraction
(HFmrEF) assessed by echocardiography 40-50%.
3. group III: Heart failure patients with reduced ejection fraction
(HFrEF) assessed by echocardiography ≤ 40%.
4. group IV: Apparently healthy persons with age and gender matched.
All participants were subjected to detailed history, full clinical
examination, liver function tests, kidney function, lipid profile, complete
blood count, HbA1c, pro BNP and assessment of expression of microRNA19b, microRNA-21and microRNA 208aby real time PCR technique.
The obtained results were as follow:
● There were no statistical significant differences between the studied
groups as regard age and gender (P<0.05). However, there were
statistically significant difference between the studied groups as regard
HTN and diabetes (P value <0.01, 0.004 respectively).
● Ejection fraction was significantly reduced in (HFrEF) patients and in
(HFmrEF) patients than (HFpEF) patients (P< 0.001).
● Total cholesterol, LDL-C and triglycerides were significantly higher in
HF groups than controls (P<0.05). However, HDL-C level was
significantly reduced in HF groups than controls (P <0.001).
● Hemoglobin level was significantly lower in HF groups (P<0.001).
However, there were no significant differences regarding white blood
cell count and platelet count (P> 0.05).
● ALT, AST, urea, creatinine and HbA1c were significantly higher in HF
groups than controls (P<0.001).
● There was statistically significant difference between the studied
groups regarding pro BNP (P<0.001), as HFrEF group was
significantly higher than HFmrEF patients, as well HFmrEF group was
significantly higher than HFpEF group.