الفهرس 
IntroductionOnly 14 pages are availabe for public view
 |  | from | 111 |  |  |
| | | from | 111 | | |
Abstract
Breast cancer (BC) is an important cause of mortality globally. Chemotherapy is used as a preventive treatment against BC; however, this usage has many burdensome effects. Thus, the era of natural therapy has gained popularity in recent years due to its safe impact on normal cells.
Green tea (GT), one of these essential herbal medicines, is an excellent source of antioxidants containing polyphenols (PP) and compounds called catechins, including epigallocatechin-3-gallate (EG), which makes them attractive sources for research into potentially novel therapeutics. In the current study, green tea extracts (PP and EG) were chosen to study their efficiency, either individually or encapsulated in nanoparticles. They induced significant cytotoxicity in breast cancer MCF-7 cells, more so than MDA-MB-231 cells.
We measured entrapment efficiency (EE) by using dialysis technology. The particle size (PZ), zeta potential (ZP), and polydispersity index (PDI) of all nanoparticles were measured by photon correlation spectroscopy (PCS) with a Zeta Sizer.
The cytotoxicity test was done to examine the cytotoxic effects of different concentrations of PP and EG on the cancer cell lines MCF-7 and MDA-MB-231. The proliferation of
MCF-7 and MDA-MB-231 cells was determined using MTT (3-(4,5-dimethylthiazol-2-yl)-2,5-diphenyltetrazolium bromide); besides, we determined the half-maximal inhibitory concentration (IC50) and fold change (FC) of the drug. The flow cytometer analysis showed the quantitative analysis of different degrees of early and late apoptosis as well as necrosis, which happened as a result of the effect of the different concentrations of drugs on MCF-7 and MDA-MB-231 cells by using the FITC Annexin V and Propidium Iodide (AnnV-PI) kit following the manufacturer’s protocol.