الفهرس 
1. IntroductionOnly 14 pages are availabe for public view
 |  | from | 120 |  |  |
| | | from | 120 | | |
Abstract
Salmonella spp. was the most prevalent pathogen causing meat diseases whereas causing many types of diseases on human.
The present study was planned to observe bacteriological studies on Salmonella spp. in raw meat products. The studies were carried out on 200 samples which collected from different markets on Port Said and transmitted to a laboratory for a bacteriological examination. The samples were taken from raw meat products and allowed to bacteriological and biochemical studies at animal health research institute, Port Said branch. Seven Salmonella isolates isolated from examined raw meat products carcass with a prevalence rate of (3.5%).
In this study, seven Salmonella isolates were biochemically a serologically identified into: two S. Anatum, one S. Enteritidis, two S. Hato and two S. Lamberhurst with a percentage of (28.6%), (14.3%), (28.6%) and (28.6%) respectively.
The antibiogram of the isolated Salmonellae revealed that, tested isolates showed resistance to Amoxycillin, doxycyclin and Erythromycin by a percentage 100 % and sensitivite to chloramphenicol, ciprofloxacin and gentamicin by a percentage 100%.
In conclusion, this study confirms the role of animal -based foods as areservoir of multi-drug resistant Salmonella and under scores the need of continuing surveillance of food-borne zoonotics pathogens along the food chain.
The identification of Salmonella isolates was confirmed by (PCR) Polymerase Chain Reaction, seven isolates were positive for invA genes giving amplification of 284 bp fragments. The same thing for the seven isolates were positive for stn genes giving amplification of 617 bp fragments While were positive for csgD gene giving amplification of 651 bp, also were positive for ompA genes giving amplification of 1052 bp fragments. Finally, seven isolates were positive for ompF giving amplification of 519 bp fragment.