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العنوان
Automated Identification of Anaerobic Infections /
المؤلف
El-­Deeb, Douaa Raafat.
هيئة الاعداد
باحث / دعاء رأفت أحمد الديب
مشرف / أمينه مصطفى عبدالعال
مشرف / صلاح عبدالفتاح أغا
مشرف / ميساء السيد زكي
مناقش / سهير عبدالرحمن محمد صالح
مناقش / محمد يوسف الجندى
الموضوع
Anaerobic Infections - Diagnosis.
تاريخ النشر
2003.
عدد الصفحات
262 p. :
اللغة
الإنجليزية
الدرجة
الدكتوراه
التخصص
أمراض الدم
تاريخ الإجازة
01/01/2003
مكان الإجازة
جامعة المنصورة - كلية الطب - Department of Clinical Pathology
الفهرس
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Abstract

There was insignificant difference in the presence of fever in all types of infections while there was significant increase in total leukocytic count in anaerobic infections than mixed and aerobic. There was significant higher resistance to previous antimicrobial, therapy in anaerobic infections than mixed and aerobic. There was significant increase in foul smell and blackish coloration in anaerobic than mixed and aerobic. Pure anaerobes were found in 16/130, anaerobes mixed with aerobes in 45/130 and aerobes in 69/130. There was no significant difference in the sensitivity of schaedler, brucella Columbia blood agar, trypcas soya and bile esculin agar with strong agrrement between schaedler and brucella, columbia and trypcase soya but weak agreement between schaedler and bile esculin agar. The isolated 61 anaerobic organisms by automated vitek system were 18 Bacteroides, 4 Fusobacterium, 4 Prophyromonas, 3 Veillonella, 3 Actinomyces, 8 clostidium, 19 GPAC and there were 2 unidentified organisms. The isolated 53 anaerobic organisms by manual API 20A were 12 Bacteroides, 4 Fusobacterium, 2 Prophyromonas, 3 Veillonella, 3 Actinomyces, 8 clostridium, 14 GPAC, and there were 7 unidentified organisms. Peptostreptococcus magnus was the commonest isolate in pus samples while B. thetaiotoamicron was the commonest isolate in blood. B. fragilis was the commonest isolate in gastrointestinal and pleuropulmonary infections. Cl. perfringens and Cl. tetani were the predominant isolates in wound infections. Pept. micros was the commonest isolate in CNS while pept. magnus was the predominant in head & neck, musculoskeletal and gynecological infections. The commonest anaerobic isolate was B. fragilis in appendicular abscesses, B. fragilis and pept. magnus in liver abscesses, pept. anaerobius in subphernic abscesses, B. thetiatoamicron & pept. niger in rectal abscesses and Prophyromonas assocharolyticus in intraabdominal infections. The commonest isolates in neck abscesses were F. necrophorum, pro. assacharolyticus and pept. assacharolyticus while in sinusitis and otitis media were pept. magnus and micros. The predominant organisms in dental abscesses were pro. gingivalis while in submandibular abscesses were pro. gingivalis and pept. anaerobius. The commonest isolates in lung abscesses were B. ureolyticus, B. thetiotoamicron, B. eggerthii and A. israelii while in empyema, B. fragilis was the commonest. In the partholin gland and vulvo-vaginal abscesses the commonest organism was pept. magnus while in ovarian abscess pept. micros was isolated. In brain abscesses, pept. micros & Cl. septicum were the predominant while in subdural abscesses F. nucleatum was the isolated organism. The commonest isolated aerobic and facultative isolates were Staph. aureus followed by E coli. There was an association between B. fragilis, B. thetaiotoamicron and Staph. aureus also between B. fragilis, pept. micros, pept. magnus and E. coli. It has been shown to be synergestic. The sensitivity of the vitek system and manual API 20A were 95.7%, 86.3% respectively with a strong agreement between the two methods. Fusobacterium, Prophyromonas and Veillonella species exhibite the highest sensitivity to metronidazole followed by Bacteroides, GPAC and clostridium while the highest resistance were observed in A. israelii.